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Detection of Human Paxillin by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 μg/mL of Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Paxillin in Detroit 551 Human Cell Line. Paxillin was detected in immersion fixed Detroit 551 human embryonic skin fibroblast cell line using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Paxillin in HUVEC Human Cells. Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips. View our protocol for Fluorescent ICC Staining of Cells on Coverslips